3MUC1gRNA2gRNA1553Papp105 cm/s)Papp105 cm/s)図1 内因性MUC1ノックアウトMDCKⅡ細胞の樹立および各種薬物の細胞膜透過性に対するMUC1の影響MUC1cMUC1cABCB1-actinP-gpMDCKb. MDCKcMUC1-KOMDCKMDCKcMUC1, cABCB1-KO154ATGDNAPCR(efflux ratio)3.493.742.332.981.791.561.171.131.211.204.104.980.210.750.880.94(efflux ratio)0.881.701.202.161.911.750.831.10 Rho123 PTX Griseofulvin Antipyrine FD-4 5-CF CDCFDA SN38mean ± S.E. (n = 3~12). *p < 0.05, **p < 0.01 (vs Mock, Student’s t-test).c. MDCKcABCB1-KO Rho123 PTX Griseofulvin Antipyrinemean ± S.E. (n = 3~12). *p < 0.05, **p < 0.01 (vs cABCB1-KO, Student’s t-test). Mock cMUC1-KO Mock cMUC1-KO Mock cMUC1-KO Mock cMUC1-KO Mock cMUC1-KO Mock0.61 ± 0.07 1.29 ± 0.31 * 1.51 ± 0.22 2.52 ± 0.56 5.19 ± 0.56 9.35 ± 1.76 * 4.37 ± 0.18 4.34 ± 0.29 0.14 ± 0.03 0.10 ± 0.03 0.51 ± 0.10 cMUC1-KO 0.46 ± 0.09 Mock cMUC1-KO Mock cMUC1-KO 0.19 ± 0.04 0.08 ± 0.01 0.77 ± 0.07 0.69 ± 0.10cABCB1-KO 0.85 ± 0.29cMUC1, cABCB1-KO 1.13 ± 0.25cABCB1-KO 1.53 ± 0.42cMUC1, cABCB1-KO 2.15 ± 0.10 *cABCB1-KO 6.71 ± 0.14cMUC1, cABCB1-KO 9.39 ± 0.71* 6.30 ± 0.22cABCB1-KOcMUC1, cABCB1-KO 5.22 ± 0.37 2.14 ± 0.26 4.83 ± 0.87 ** 3.53 ± 0.49 7.52 ± 1.34 ** 9.31 ± 0.51 14.58 ± 3.88 5.13 ± 0.11 4.92 ± 0.09 0.17 ± 0.02 0.12 ± 0.01 2.09 ± 0.75 2.29 ± 0.53 0.04 ± 0.01 0.06 ± 0.00 0.68 ± 0.16 0.65 ± 0.21 0.75 ± 0.04 1.92 ± 0.40 * 1.83 ± 0.05 4.65 ± 0.77 ** 12.84 ± 0.48 16.44 ± 0.96 5.25 ± 0.79 5.75 ± 0.55a.
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